Your English writing platform
Discover LudwigSuggestions(1)
Exact(3)
After 48 h had elapsed, the samples were placed into a Beckman Avanti® J-26 XP centrifuge and spun down at 2000 rpm (973 rcf) for 10 min utilizing a JS 5.3 rotor in order to remove particulates.
Secondary antibody incubation was performed at room temperature for 30 min utilizing a horseradish-peroxidase (HRP -labeled rabbit IgG on rodent polymer (Biocare Medical, Concord, CA), and staining was visualized witHRP -labeled Fast DAB tablets, Sigma) forabbitIgGat ronm temperodent.
The column was equilibrated in 85% A/15% B prior to the injection of the sample, and upon injection this composition was then changed to 60%A/40%% B over 30 min utilizing a linear gradient followed by changing to 50%A/50%% B over the next 10 min, and then, the concentration was returned to 85%A/15%% B over the final 10 min.
Similar(57)
A high polymer yields of above 95% were achieved at 160 °C for 15 min utilizing ɛ-caprolactam magnesium bromide as a catalyst.
The PCR was performed at 95°C for 5 min as the initial step and then for 30 cycles of 95°C for 30 s, 55°C for 30 s, and 72°C for 2 min, followed by a final extension of 1 cycle of 72°C for 7 min, utilizing the following primers; mNoc3UTRF 5'-CTAGATCACAAGCGTCTTAACCAGGG-3', mNoc3UTRR 5'- TCTAGAACTTTATAAATAAGATATTTACCATTTTACCACTGG-3'.
Transthoracic echocardiography was performed in hemodiluted rats in the supine position at baseline and within 15 to 30 min following hemodilution utilizing a high-frequency ultrasound system (Vevo 2100, MS-250 transducer, Visualsonics, Toronto, ON).
For analysis of proteins secreted in the culture medium, culture supernatants obtained after centrifugation (3000×g, 15 min) were filtered utilizing a 0.22-µm sterile low-protein binding PVDF filter unit (Millex GV, Millipore) and the serine-protease inhibitor phenyl-methyl-sulphonyl-fluoride (PMSF) was added to 1 mM final concentration.
Thus, the RAND strategy utilizing a min-cost optimization approach can be considered as the preferred method for bed assignment in the case of shared resources.
Samples were infused utilizing a Harvard Instruments Syringe Infusion Pump 22 at a flow rate of 10 ml/min.
Nipponbare utilizing a deep sequencing approach.
In brief, patients underwent leukapheresis for five hours with patients with white blood cell counts of less than 35×10/L processed at a blood flow rate of 90 ml/min utilizing a cycle volume of 1,400 ml and those with white blood cell counts greater than 35×10/L processed at a blood flow rate of 65 ml/min utilizing a cycle volume of 1,000 ml.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com