Immediately following the draw, the anticoagulated blood was centrifuged at 1100 rpm for 12 min using the Eppendorf Centrifuge 5702.
The photoresist is then removed in ultrapure acetone, and the entire wafer is etched for another 45 min using the same recipe.
The complete removal of diclofenac was achieved at 90 min using the Fenton-like system.
Approximately 10 min after finishing their second cigarette they were again interviewed for 12 20 min using the same semi-structured interview and rating scales as in the morning session.
The plasma glucose concentration was checked every 5 10 min using the glucose dehydrogenase method (HemoCue AB, Angelholm, Sweden) and held constant at 5 mmol l−1 by a variable glucose infusion (200 g l−1).
Medical technicians with few experiences can design cast within 20 min using the proposed technique.
The duration for the sequence collection was set at 180 min using the stage start option.
Once anesthetized, the rats were scanned for 90 min using the Inveon microPET scanner in full list mode.
The gels were then transferred onto PVDF membranes for 7 min using the iBLOT western transfer system (Invitrogen, Carlsbad, CA, USA) at room temperature.
To derive VT values, kinetic modelling was performed on TACs from 0 to 63 min, using the R package kinfitr (version 0.2.0,www.github.com/mathesong/kinfitr).
The measurement time was about 1 min using the surface resonance frequency, whereas measurements at 3 mm depth resulted in considerable longer acquisition times (about 5 min).
