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An anesthesiologist administered each infusion over 40 min using a Baxter infusion pump (Deerfield, IL, USA).
Immediately after lysis, nuclei were spun at 500 × g for 10 min using a refrigerated centrifuge.
Fluorescence kinetics data were collected every 3 or 4 min using a microplate reader (Synergy H1, Biotek) over 48 h.
The assays were accomplished in less than 30 min using a LightCycler in real-time mode.
Lactate levels were determined every 30 min using a Lactate ProTM 2 blood lactate meter (Arkray, Alere AB, Lidingö, Sweden 18.
Following a stabilization period of 2 3 h, the samples were collected every 20 min using a CMA/170 refrigerated fraction collector.
The beads were ultraviolet-irradiated for 60 min using a 365 nm LED array (LC-L5, Hamamatsu Photonics K.K., Hamamatsu, Japan) as described in former publications24,25.
A small volume of samples was added to the μPADs, which was photographed after 15 min using a digital camera.
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ACSF was perfused at a rate of approximately 2 ml/min using a gravity-driven perfusion system.
Aβ25 35 solution (3 μl, 6 nmol) was then injected intracerebroventricularly at a rate of 1 μl/min using a syringe pump.
The remaining acetonitrile was evaporated under mild vacuum conditions for 30 min using a rotary evaporator.
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