Sentence examples for min upon which from inspiring English sources

Samples were kept in the dark at room temperature (22 ± 1°C) for 30 min, upon which absorbance stabilized.

The filtrates were run for 30 min upon which the volume of filtrate was recorded and the filter cake thickness was measured.

The sample was incubated for 1 min, upon which the supernatant was collected.

In 15 of 76 cases (Fig 5C), the track of one or both daughter nuclei could be followed until they entered the neuronal layer (Fig. 4C and Movie S3 Figg S3A, white bars, 329±156 min), upon which discerning a possible Tubb3-mGFP expression became very difficult due to the presence of neighboring Tubb3-mGFP positive cells.

The reaction was stirred for 15 min, upon which concd NH4OH solution was added to pH 11.

Infection or LPS stimulation was allowed to proceed for 15 min upon which cells were fixed with 3% paraformaldehyde for 15 min at room temperature and counter stained with DAPI.

The reduction was carried out for 7 min at 20°C upon which the reaction was quenched by the addition of concentrated hydrochloric acid (37%, 0.1 mL).

The heating step was repeated to solubilize all triglyceride, upon which samples were centrifuged for 2 min to remove any insoluble material.

Images were acquired for up to 120 min prior to euthanasia by cervical dislocation under anesthesia, upon which the skin was surgically opened.

Performing dose-response experiments ranging from 10 ng/ml to 0.1 ng/ml IL-1 for 15 min revealed 0.5 ng/ml to be the sensitive dose of choice upon which initial IκBα degradation has only partially taken place.

However, with continued pacing beyond 10 min these subsidiary peaks disappeared, consistent with a reduction in available SR Ca upon which such events might depend.