Sentence examples for min treated with from inspiring English sources

The coverslips were removed by immersing the slides in 1 × standard saline citrate (SSC) solution at 48°C for 15 min. Subsequently, the slides were washed twice in 50% formamide in SSC, twice in 1 × SSC for 20 min, treated with RNase A (10 μg/ml) for 45 min at 37°C and then washed in 1 × SSC, 0.5 × SSC and 0.25 × SSC at 48°C for 10 min. The last wash was done in 0.25 × SSC at room temperature.

In brief, for each assay, at least 40 synchronous L4 larvae or young adults were transferred to NGM plates containing inactivated OP50 °C5 °C for 30 min), treated with 40 µM of FUdR to inhibit the growth of progeny and scored every other day.

Blank controls were prepared from supernatant derived from E.coli cultures boiled at 100°C for 10 min, treated with 5mg ml−1 asocasein and directly thereafter by TCA.

Cultures were permeabilized using 0.3% Triton X-100 for 10 min, treated with 2N HCl for 25 min, and neutralized with two washes of 0.5 M sodium borate, pH 8.5 for 5 min. After blocking in 1.0% BSA for 20 min, primary anti-MHC antibody; 1 50 (clone MY-32, Sigma) for 1 hr, cells were washed 3x, 3 min each with PBS.

ESCs were cross-linked with 1% formaldehyde for 10 min, treated with glycine at a final concentration of 0.125 M for 5 min at room temperature, and lysed in lysis buffer (5 mM HEPES; pH 9.0; 85 mM KCl, 0.5% Triton X-100) for 15 min on ice.

Reverse Transcriptase reactions were performed using Superscript II (Invitrogen) at 45°C for 90 min and reactions were stopped at 70°C for 15 min, treated with DNase free Ribonuclease for 30 min at 37 C and purified on colums (Qiagen).

An ICC analysis was also performed by fixing NPCs in 4% PFA for 20 min, treating with 0.1% Triton X-100 in PBS for 20 min and incubating with primary antibodies at 4 °C overnight before incubation in blocking solution for 1 h.

Cells were washed (PBS, 3×5 mins), treated with secondary antibody (Molecular Probes, A21244, Alexa647-conjugated goat-anti-rabbit IgG, 1∶3000, 2 hrs, room temperature in the dark) and washed again (PBS, 3×5 mins).

For heat shock, the mid-log cells were harvested, re-suspended in pre-warmed YPD media, incubated in 39°C water bath for 15 mins, treated with formaldehyde, and then stored at -80°C.

Mitochondria were incubated in medium for a total of 10 min and treated with HNE for 9 min (where appropriate).

In addition, rice roots were also pretreated with 0.1 mM CNQX for 30 min, then treated with 20 mM Glu for 30 min (CNQX + Glu).