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The oven temperature was programmed as following: 140°C initially hold 10 min, and then increased to 250°C at 7°C/min, with the final hold for 10 min, with 35.71 min total time of GCMS analysis.
Title: Lucky Kiwi and Coconut Cupcakes Author: Adapted from Foodnetwork Prep Time: 1 hr and 10 min Cook Time: 25 min Total time: 1 hr 35 min Serves: 20.
Recipe Name: Proper Position Pecan Pie Author: Dough Mamma & Cook's Illustrated Prep Time: 1 hr 15 min Cook Time: 25 min Total Time: 1 hr 40 min Serves: 2 pies or 12 mini pies Notes: I used bacon lard in my pie dough, you can substitute suet.
To measure association rates, dilutions of SEB (11 2700 nM) in 0.1 M HEPES buffer containing 1.5 M NaCl, 30 mM EDTA, and 0.5% P40 surfactant (pH 7.4, 25°C) were used at a flow rate of 50 µl/min (3 min total time).
N = 13, 18 for WT and T238A microtubules respectively, with t = 35, 72 min total time observed.
N = 24, 14 for T238S and T238V microtubules respectively, with t = 120, 43 min total time observed.
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Prep time: 2 mins Cook time: 3 mins Total time: 5 mins Serves: 2.5 cups Classic American Buttercream frosting.
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