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Bacterial cells were centrifuged at 6000 rpm for 10 min to settle down the bacterial pellet.
The 96-wells plate was centrifuged at 2000 rpm for 10 min to settle the suspended cells at the bottom.
After spending about 7 min to settle down the students and remind them about the upcoming science test, Kenny began the lesson on the human digestive system.
The suspension was then centrifuged at 1000 xg for 5 min to settle the cellular debris.
For cage activity test, mice were placed on to a grid of squares (3×3 cm) in a cage, and were allowed 5 min to settle before testing began.
Samples were centrifuged at 10,000 rpm for 10 min to settle down the culture.
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The mixture was vortexed for 1.0 min, allowed to settle for complete phase separation and centrifuged (6,000 × g/ 3 min).
The water was mixed rapidly at 100 rpm for 2 min, mixed slowly at 25 rpm for 30 min, and allowed to settle for 60 min. Turbidity and pH of the supernatants were measured immediately using a 2100N turbidimeter (HACH, Loveland, CO) and an AR20 pH/conductivity meter (Fisher Scientific), respectively.
After 3 min of rapid mixing at 100 rpm, the speed was reduced to 35 rpm for 15 min, then allowed to settle for 15 min.
Each fecal sample was diluted in sterile saline solution (1 10 dilution) with chloramphenicol (200 mg/L), vigorously homogenized for 5 min, and allowed to settle for 15 min.
73 min: Ireland attempting to settle down with some keep-ball.
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