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Fresh rabbit blood was incubated for 1 h at 37°C, the clotted blood was centrifuged at 1000 g for 20 min to separate the serum.
The samples were centrifuged at 15,000 rpm for 5 min to separate the supernatant.
The culture broth was centrifuged at 4000 rpm for 10 min to separate the cells.
Tubes were centrifuged at 2000 g for 10 min to separate the plasma.
Then centrifuged (1000 × g at 22°C) for 8 min to separate phases.
The as-prepared solution was uniformly dispersed by sonication during 2 min to separate the aggregations and then filtered.
Afterwards, the resuspended sample was centrifuged at 14,000 g for 30 min to separate the soluble and insoluble fractions.
The sample was immediately stored on ice and then centrifuged within 30 min to separate out plasma.
The fermentation broth was centrifuged at 10,000 rpm for 20 min to separate the precipitate and supernatant.
The strain cell lysate was centrifuged (8000g, 4 °C, 30 min) to separate the precipitate and soluble parts.
At each time points, the blood was collected and centrifuged (2000 rpm for 10 min) to separate the plasma.
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