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Briefly, 0.50 g frozen soybean leaves were homogenized at 4 °C in 5.0 mL of 50 mM phosphate buffer (pH 7.0) containing 1.0 mM EDTA, 2% NaCl and 1% polyvinyl pyrrolidone and centrifuged at 15,000×g for 15 min to select supernatant as the enzymatic extract.
Birds were given 1 min to select one of the mealworms before both dishes were removed from the cage.
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Click on the "Min" tab to select 10%.
The homogenate was centrifuged at 12,000×g for 30 min at 4 °C to select supernatant using as extract.
0.50 g of frozen soybean leaves was homogenized in 3 mL of 0.5% thiobarbituric acid (TBA) in 20% TCA and then centrifuged at 12,000×g for 20 min at 4 °C to select the extract.
Prior to morphology studies and drug treatment, cultures were synchronized with 5% D-sorbitol [ 42] for 5 min at 37°C to select for parasites in the ring stage.
Then it was placed at room temperature until be cooled and about 2 g of feces were inoculated into it and was placed at 65°C in a water bath for 10 min in order to select bacterial spores and was incubated in an anaerobic incubator (Finetech, Germany) at 37°C for 7 days.
Preliminary experiments had demonstrated that preexposure for 15 min to those selected conditions resulted in optimal heat resistance (Figure S1 in the supplementary material).
The D-value was determined from the inactivation kinetic curve given by the equation: t = D × (log No-log Nf) = D × n [ 1], where D = D-value (min) at specified conditions, No = bioburden of the chosen bacterium as the BI; Nf = surviving population after an exposure time, t (min), to the selected disinfectant and n = (log No-log Nf) = log10 reduction of a bioburden.
The time to perform a measurement is 2 3 min (one minute is spent to select the correct endoscope).
Such a responsible position at such a young age induced Time magazine to select Min in 1994 as one of its top fifty future leaders.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com