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Island 2 requires 325 min to restore and subsequently followed by resynchronization between the two islands.
Next, the plates containing the cells on the membrane were dried (88°C for 150 min) to restore membrane hydrophobicity.
These tissues were then washed over 60 min to restore basal tone before being contracted by PE, to approximately 80% of the maximum PE-induced response.
Cells were then shifted to 32°C for 90 min to restore transport from the TGN and analyzed by immunofluorescence (Wakana et al., 2012).
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It seemed Massimo Luongo was set to be the name etched indelibly into the annals of Australian sport with his goal on the stroke of half-time, only for Son Heung-min to restore parity a minute into injury time just as Australia could taste victory.
Based on the modelling, 1 min of hot water usage requires approximately 10 min of heating to restore the temperature set by the thermostat.
Fluid resuscitation was initially set at about 2 ml/kg per min over the first 10 min. If this approach failed to restore arterial blood pressure, then fluid resuscitation was enhanced to about 8 ml/kg per min. We used a combination of Ringer's lactate and gelatine solution for fluid resuscitation, because it is the usual strategy in Europe.
The assay medium was then gently mixed again for 3 5 min between each rate measurement to restore normal oxygen tension and pH in the microenvironment surrounding the cells.
Resuscitation was made in vivo by reinfusion of the shed blood to restore norm tension for 30 min.
Synaptosomes were incubated for 30 40 min at 37°C in order to allow them to restore transmembrane ion gradients before further use in amino acid release experiments.
After 30 min of MCAo, the suture was carefully removed to restore blood flow.
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Justyna Jupowicz-Kozak
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