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After thawing, the lysates were incubated at 72 °C for 20 min or 75 °C for 5 min to release small RNAs for reaction.
Samples were heated at 80 °C for 15 min to release protein-bound ET, then clarified by protein precipitation with 500 µl of cold acetone overnight at −20 °C.
In particular, conventional TP analysis systems require an indispensable pretreatment step, in which the fluidic analyte is heated to 120 °C for 30 min to release the dissolved phosphate, because many phosphates are soluble in water at a standard temperature and pressure.
It was necessary to allow the slurry to stand for 5 min to release the entrapped air before all measurements.
About 300 mL of the extracted DNA containing 2.0 M of NaCl and 1 mM of AQMS was sonicated for 15 min to release the DNA breaks.
Immediately, the stretched samples were put in the ice water bath (2 3 °C) for 5 min to release the stretch, and the length was measured asl2.
Similar(29)
Oil palm empty fruit bunch (OPEFB) was hydrolyzed with dilute sulfuric acid (6% v/v; 8 mL acid per g dry OPEFB) at 120 °C for 15-min to release the fermentable sugars.
Cells were then shifted to the indicated temperature for 30 min. To release from G1 arrest, the cells were washed 3 times with warm water and then resuspended in pre-warmed medium at the indicated temperature.
At the end of the incubation period, the macrophages were washed twice with PBS and lysed with 0.01% (v/v) SDS in PBS (30 min, RT), to release internalized parasites.
The membrane was then dissolved in methylene chloride for 10 min to completely release the microtubes.
53 min: Gyan attempts to release Tagoe on the right wing but over-hits his pass.
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CEO of Professional Science Editing for Scientists @ prosciediting.com