Cannulas were then left in place for 5 min to prevent backflow.
Molds were then centrifuged at 3,000 rpm during ~1 min to prevent air bubbles formation and hardener.
The electrode was cleaned with 14% ammonium hydroxide for 2 min and MilliQ water for 1 min to prevent cross-contamination.
Following treatment, coverslips were washed with PBS and fixed in 2% paraformaldehyde for 15 min at RT. Coverslips were then washed 3 times with PBS and blocked with 2% BSA (Sigma) for 30 min to prevent non-specific binding.
Samples were centrifuged (4000 rpm, 15 min, 4 °C) and the supernatant was treated with RNase A (100 μg ml−1, 2 3 min) before centrifugation (13,500 rpm, 1 min) to prevent RNA-mediated carryover.
This was provided in a large bowl, with the pasta topped up every 10 min to prevent visual feedback of food consumed and any tendency to finish the provided portion.
The aim of this study was to assess the effectiveness of the unipedal standing balance exercise for 1 min to prevent falls and hip fractures in high-risk elderly individuals with a randomized controlled trial.
After development, the sample was hard-baked at 95°C for 5 min to prevent photoresist deformation during plasma etching.
Once separated, milled bran was heat stabilized at 110 °C for 6 min to prevent rancidity during storage.
All glassware and materials were washed and sterilized in the autoclave at 121 °C for 15 min to prevent any influence by other microorganisms.
Prior to SDS-PAGE analysis, the enzymes were treated with 25 mM phenylmethane sulfonyl fluoride (PMSF) dissolved in methanol for 30 min to prevent autolytic degradation.
