Sentence examples for min to increase from inspiring English sources

Prior to cell passaging, cells were subjected to a brief pre-treatment with blebbistatin (>5 min) to increase cell viability, treated with Accutase for 7 min, triturated to a single-cell suspension, quenched with an equal volume of mTesR1, pelleted at 80g for 5 min and resuspended in mTesR1 containing blebbistatin.

Then, the sample was heated at 170 °C in air for over 30 min to increase the adhesion between the graphene and the substrate.

Further chemical etching was conducted in 5 wt% phosphoric acid at 30 °C for 15 min to increase the pore diameter of the alumina mask.

Then, the samples were treated in KOH 0.1 M for 3 min and HNO3 0.1 M for 10 min to increase the density of surface hydroxyl groups.

After staining, the slices were immersed in 10% formalin for 20 min to increase the contrast between the healthy and the infarcted myocardium.

The suspension was sonicated for 30 min to increase the solubility of the lipid and to promote the disruption of large multilamellar vesicles (LMV) that were presumably present in the suspension.

The 3 minute recommended exposure was increased to 10 mins to increase the selection power.

PCR amplification was 94°C for 3 min, 35 cycles of 94°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec, then 72°C for 3 min. To increase the specificity of gene amplification, primer sets were designed with use of Vector NTI (v9.0) with the 3'UTR sequence for each OsADF gene, except for OsADF7, OsADDF8a and OsADF8b, whose primers matched the coding region.

On the second sampling day, sampling was conducted for 60 min in order to increase yield.

In practice, we used a two-phased min hash strategy to increase specificity (see Methods).

During the experiment substrate with high density, collected at 70 min, led to increased calcification of their matrix.