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The slide was held horizontal at 4°C for 30 min to improve adherence.
After metallization, the samples were heated at 500°C for 3 min to improve the electrical properties of the contacts.
The sample was subsequently annealed at 150 °C for 30 min to improve both the quality and the conductivity of the silver films on the PDMS substrate.
Lysozyme was added to the bacterial cells at a final concentration of 10 μg/mL and the cells were then incubated at 37 °C for 30 min to improve transformation efficiency.
After the substrate was heated at 50°C for 20 min to improve the quality of the PMMA, a single ZnO MR was transferred to the prepared p-GaN substrate and crossed the boundary with the p-GaN and PMMA.
A 1-μm aluminum back-side electrode was coated by e-beam evaporation and annealed at 600 °C under H2 ambient for 10 min to improve the contact between Si and Al.
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The flow rate was 1.0 ml/min and the eluent was MilliQ-water for 35 min followed by an increase to 200 mM NaOH for 10 min and a final equilibration with MilliQ-water for 10 min. To improve detection post-column mixing with 200 mM NaOH at 0.5 ml/min was used.
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The obtained layer thickness was below 1 μm for deposition times of 2 min. To improve the adhesion of the deposited layers on the substrate materials, sputter cleaning by energetic ions was used during the initial phase of the process.
By deploying M antennas at transmitter, and N antennas at receiver, MIMO takes advantage of the spatial multiplexing gain of min(M,N) to improve the date rate.
Based on the results of the pilot study, incubation times were changed from 2 x 30 min to 2 x 60 min in order to improve sensitivity.
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