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After transfer, the membrane was washed with 8% acetic acid for 20 min to fix the proteins, and then rinsed with water before air-drying.
We find that at least 10 min of observations are required for most receiver types to reliably fix about 90% of wide-lane ambiguities corresponding to high elevations, and over 20 min to fix about 90% of those corresponding to low elevations.
200 μL of 99% methanol was added to each well for 15 min to fix biofilm.
Subsequently, 4% paraformaldehyde was added for 30 min to fix the cells.
Subsequently, the sample was heated on a hot-plate at 120°C for 2 min to fix the MWCNTs.
However, although software installation and use was little problem, there were difficulties with workspace and webcam set-up, establishing facial recognition parameters and maintaining a lengthy live video-feed: "….the invigilator stopped the exam because the camera feed wasn't coming through took 40 min to fix, 40 min of lost time.
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Wash slides with PBS twice at RT for 5 min. Incubate slides in 0.1% NP-40/2 x SSC at 37oC for 30 min. Soak slides in 70%, 85 % and 100 ethanol at RT for 2 min each (to fix tissue).
After the films were washed three times with PBS, they were immersed in 1% glutaraldehyde in PBS for 120 min at 4°C to fix the adhered platelets.
Upon the addition of 50 μl of cold TCA (10% TCA) the assay was terminated and incubated for 60 min at 4°C to fix the cells.
Ovaries were dissected in PBS for no longer than 20 min prior to fixing.
At t = 23.5 h, cells were incubated with 10 μM Hoe and either 1 μM MitoTracker™, 10 μM Mitosox, 10 μM H2-DCFAM, 10 μM HPF, or DMSO for 30 min prior to fixing in ice-cold PFA.
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