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Cell pellets were resuspended in 1 ml methanol and vortexed for 5 min to extract curcumin in methanol fraction.
When run on 80 processors, this version of PPP took only 67 min to extract probes, while some 87 h were needed by the sequential version of PPP.
After required period of incubation, 50 mL of sterilized distilled water was added to the fermented substrate, and further incubated in an orbital shaker at 150 rpm for 30 min to extract the enzyme.
The blood in the plain tubes was immediately centrifuged at 3000 revolutions per minute for 10 min to extract serum which was stored at −20 °C and used within 12 h for biochemical assays.
After washing the cells twice with pre-cooled PBS (pH 7.4), ice-cold cell lysis buffer was added to the cells, which were then placed in an ice bath for 15 min to extract the protein.
The quality loss is due to the artifacts introduced by the block-based MC process, as opposed to optical flow mostly, but also due to the saliency model performance: the GBVS model has the best results, but unfortunately at the expense of processing time (it takes about 1 min to extract the saliency map).
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The remaining gel pieces were extracted with a 60% acetonitrile and 0.2% TFA solution for 1 h with a 5 min sonication to extract remaining organic peptides.
Then, the strips were cut into 44 equal pieces along pH gradient, and each piece was consecutively incubated in 100ul of 0.1% FA, 0.1%FAcetonitrileitrile (ACN), and 0.1%ACN80%ACN for 10 min each to extract labeled peptides from gel.
Then, reconstituted cells were homogenized with a Dounce glass homogenizer, and the resultant cell homogenates were subjected for centrifugation at 10,000 × g for 10 min (4°C) to extract the nuclear and mitochondrial organelles.
An extraction time of 10 min was enough to extract the ethers and BTEX.
With these conditions, an extraction time of 5 min was sufficient to extract MTBE.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com