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In addition to this, experimental mode soundings are scheduled at 5 min to each hour at Wakkanai.
The procedure was repeated at same sites with injection of 1 ml of 0.5% bupivacaine followed by 1 ml of 95% ethyl alcohol after 10 min to each site, because injection of alcohol alone might be very painful.
Then fresh medium containing 50 µg of gentamicin per ml (Ameresco, Solon, OH, USA) was added for 30 min to each well to kill extracellular bacteria and the number of intracellular bacteria was determined as a control (referred to as the zero point).
delivered a mini-lecture (15 min) to each of the 15 discussion sections in the week following the midterm exam.
At each time point 5, 20, 35, and 50 min, 3 mL of distilled water was added for 5 min to each well to lyse red blood cells (RBC) not trapped within the clot.
In all cases, SiHa cells were exposed for 30 or 60 min to each drug and incubated for 24 hours after drug treatment to allow time for DNA repair and concomitant loss of γH2AX foci.
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They took between 25 and 40 min to finish each test.
We found that it took, on average, 15 20 min to complete each block version of the questionnaire.
Subjects used near vision correction, if needed, and were given 2 min to complete each of the four test boxes.
Participants were given ∼5 min to complete each assessment item.
Participants were given 3 min to complete each Activity (task duration: 6 min).
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