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The mixture was heated in boiling water for 10 min to develop the pink colour.
For example, the OraQuick test takes a minimum of 20 min to develop, but may be read within a 20 40 min reading window.
2.0 ml of anthrone reagent (0.05 g anthrone per 100 ml of 72%H2SO44) was added and boiled for 10 min to develop a color and then chilled again.
The mixture was heated in a boiling water bath (95 to 100°C) for 10 min to develop a pink color, cooled with running tap water, and centrifuged at 3,600 × g at 25°C for 20 min.
The mixture was heated in boiling water for 10 min to develop a pink color, cooled with running tap water, sonicated for 30 min, followed by centrifugation at 5,000×g at 25°C for 10 min. The absorbance of the supernatant was measured at 532 nm.
The mixture was heated in a boiling water bath (95 100 °C) for 10 min to develop a pink colour, cooled with running tap water and then sonicated for 30 min, followed by centrifugation at 5000×g at 25 °C for 10 min.
Similar(40)
As stated earlier, the alloy specimens were heated prior to the application of the adhesives to temperatures of 100, 200 and 300 °C for 60 mins to develop an oxide layer on each of the specimens.
Reid's office said the senator sold off 110 acres with eight claims to Nevada Milling and Mining to develop a gold mine, and two additional mining claims not included in the sale price were sold to American Capital Energy.
There was a trend for indolent MIN lesions to develop earlier than lesions that went on to progress to invasive cancer, to be closer to the lymph node and to have a lower growth rate compared with progressive MIN (Table 2).
Following phagocytosis, Hoechst+, PI− rods retained within a phagosome (for up to 30 min) were observed to develop PI nuclear staining, followed by a gradual (≈10 min) disappearance of nuclear staining (Fig 5C), indicating membrane permeabilization and cellular breakdown within the phagosome.
Samples were incubated 5 10 min for color to develop.
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