Sentence examples for min to determine from inspiring English sources

It generally takes watching 5 min to determine that these Hamlets are different.

The samples were pack carburized at 850 °C for 30 and 60 min to determine the surface properties.

C2C12 myoblasts (ATCC; passage 5 9) or HEK293 cells were used for all experiments and maintained in DMEM containing 10% FBS and 1% Penicillin/Streptomycin. C2C12 myoblasts underwent serum starvation for 2 h, followed by treatment with 5 μM anisomycin for 30 min to determine the effect of JNK activation on SMAD2-linker phosphorylation.

Five microliters (5 µL) of the amplified products were run on agarose gel electrophoresis at 120 V for 15 min to determine the quality of the products.

The elution test was carried out at a specific S/L ratio and at varying time (from 5to9090 min) to determine the effect of elution time.

Tail vein blood samples were collected before, and after glucose loading at 20, 60 and 120 min to determine glucose concentrations using an Accu-Check glucometer (Roche Diagnostics, Manheim, Germany).

PCR cycling conditions were followed by 25 cycles of 94°C for 1 min, 55°C for 2 min, 72°C for 3 min. To determine the sensitivity of the PCR, cells were cultured in YM medium, and the cell density was measured.

The 90-s stimuli were separated by 5 s, and the pause between the two stimulus runs was 5 min. To determine the noise level, we recorded 2 min of MEG without stimulation.

The ghrelin receptor PCR primers were: sense, 5'-TCTTCCTTCCTGTCTTCTGTC-3' antisense, 5'-TCTTCCTTCCTGTCTTCTGTC-3' antisenseondition was 95'-TCTTCCTTCCTGTCTTCTGTC-3' antisense°C 30 s 30 cycles, 72 °C 10 min. To determine the cell growth percentage, the MTT assay was operated.

After being enrolled of all the components, reactions were shaken at 220 rpm at 37°C for 30 min. To determine the amount of n-hexane produced, a sample of the headspace was collected using a gastight sample lock Hamilton syringe and analysed by Shimadzu GC-2010 with DB-5 column.

At the end of treatment, Cells were fixed with 4% formaldehyde in PBS (pH 7.4) for 15 min. To determine the autophagic response, cells are inspected at 60× magnification for total number of GFP-LC3 puncta.