Sentence examples for min to destroy from inspiring English sources

A 150 μl 0.6 N NaOH was added to above mixture and boiled for 10 min to destroy reducing sugars, and then chilled again.

The serum contains several proteins, bovine serum albumin, fibronectin, vitronectin, etc. FBS is heat inactivated (56°C, 25 min) to destroy the immunological components yet preserve the proteins.

Except fermented milk, 10 mL of each raw milk sample was heated at 80 °C for 10 min to destroy natural inhibitors lysozyme and lactoferrin (Hillerton et al. 1999).

The soil had been sieved at 2 mm mesh size, and autoclaved at 121 °C for at least 15 min to destroy any natural enemy of grubs or EPNs as well as any potential natural population of EPNs.

In brief, the sections were exposed to 0.5% hydrogen peroxide in methanol for 30 min to destroy endogenous peroxides activity after deparaffinization and rehydration.

The sections were first treated with 0.01% H2O2 in methanol for 20 min to destroy endogenous peroxidases and then incubated for 1 h in 0.1% Triton-X 100 and 1.5% normal goat serum to minimize nonspecific labeling.

The samples were autoclaved (5 min, 121°C) to destroy the tissues and measured after cooling to room temperature (Cmaximum).

The vials were then capped, vortexed, and incubated at room temperature for 25 min. Then 9 µl of 12% acetic acid in hexane were added to each sample and left at room temperature for another 25 min in order to destroy all excess trimethylsilyldiazomethane.

Cells in triplicate were exposed to Dox (5  μg/ml; 3 days) in the presence or absence of the ODNs (30  μM) in a medium with 10% heat-inactivated FBS (30 min at 65 °C to destroy nuclease activity).

After the addition of the plasticiser at the level of 50% of polysaccharides total solids, the biopolymer-glycerol dispersions were heated to 80 °C for 30 min to allow full dissolution and hydration and to destroy any residual microflora.

After AHP pretreatment, the biomass suspensions were neutralized to approximately pH 7 with concentrated HCl, treated with catalase to destroy residual H2O2, heated at 90°C for 15 min to inactivate the catalase, and lyophilized to dryness [ 20].