Suggestions(2)
Exact(6)
In the analytic method, the integration time is set to be approximately 1 min to decrease the number of phase variations.
The ITO electrode was immersed in the PBS containing 0.4 μM aptamer, followed by rinsing with PBS and dried with N2 again, then dipped in the BSA solution for 30 min to decrease the non-specific binding.
In order to remove an oxide layer of the substrate, the samples were etched in a 1 M NaOH solution for 3 min. The Al sheet was electropolished in a mixture of perchloric acid and ethanol with a value ratio of 25:75 for 3 min to decrease the roughness of the substrate.
After an initial decrease, that accompanied MPF activation and reached a minimum at the MPF activity peak (Fig. 1 B; 26 min; Fig. 1 C; 28 min), the cdk1-phopsho-tyr-15 signal transiently increased just around the time cyclin degradation begun (Fig. 1 B; 28 min; Fig. 1 C; 30, 32 min), to decrease again when substantial cyclin degradation was achieved (Fig. 1 B; 32 min; Fig. 1 C; 34 min).
Cells preincubated with 10% bovine serum albumin (BSA; Sigma-Aldrich, St . Louis MO, USA) in PBS for 20 min to decrease nonspecific antibody binding were incubated for 60 min with a 1 : 100 dilution of primary antibody (anti-Cav-1 antibody) in a solution containing 1% (v/v) BSA in PBS, and washed three times for 10 min each with PBS.
The criteria for abnormal yawning of Singer et al. (2007) were adopted and expanded from 2 yawns/10 min to ≥3 yawns/15 min to decrease the likelihood that 2 subsequent yawns by chance were counted as one episode with abnormal yawning.
Similar(54)
The transcript levels of ethylene biosynthetic genes, ACS6, ACO3, and ACO4, were also promptly accumulated at 15 min and began to decrease at 30 min (Fig. 5).
The 8‐oxo‐dG levels in the adipose tissues of KK‐ A y and Min mice tended to decrease following the 500 mg/L apocynin treatment (Fig. S3).
Then, the slides were incubated in 1% normal rabbit serum for 30 min at RT to decrease the nonspecific antibody binding.
Change of powder pore structure (Figure 12) shows that increase of decomposition time from 30 to 60 min led to decreasing pore volume with mean size 20 to 50 nm and rising pore volume with size 3 to 4 nm.
After about 30 min, the pH ceased to decrease indicating complete dissolution.
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