Sentence examples for min to block from inspiring English sources

And sequentially treated with 2.5% normal horse serum for 20 min to block nonspecific labeling.

Subsequently, the sections were immersed in 3% hydrogen peroxide for 10 min to block the endogenous peroxidase activity.

After centrifugation, the cells were resuspended in 5% donkey serum in 0.3% Triton-X for 30 min to block and permeabilize the cell membrane.

They were then treated with 0.3 % H2O2 for 15 min to block endogenous peroxidase.

Samples were incubated with 10%% goat serum for 30 min to block non-specific binding of the antibodies.

Ethanolamine (1M, pH8.5) was injected for 7 min to block the remaining activated groups.

The cells are left at RT for 10 min to block aspecific sites on the cell surface.

An excess amount of sulfo-NHS acetate was introduced and kept for 15 min to block the unreacted amine groups.

Briefly, cells were resuspended in PBS + 5% FBS, human IgG (1∶100) was added and cells were incubated on ice during 45 min to block unspecific binding.

Tissue sections were incubated with 1% goat serum or bovine serum albumin (BSA) at room temperature for 30 min to block non-specific binding.

For immunohistochemistry, sections were treated with 1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) for 30 min to block nonspecific antibody binding.