Sentence examples for min to avoid from inspiring English sources

Cannulas were left in place for another 5 min to avoid backflow.

After injection, the micropipette was kept in place for 5 min to avoid backflow of the injected vector during micropipette retraction.

After the recent session, the experimenter had a conversation with the patient for around 15 min to avoid rehearsal of items in short-term memory.

In the this research, chitosan scaffolds are cross-linked with hydrothermal treatment with autoclave sterilization time of 0, 10, 20 and 30 min, to avoid the application of the traditional chemical toxic materials.

Total RNA was incubated at 70 °C for 10 min to avoid secondary structures.

When the infusion rate of insulin was changed, blood glucose concentrations were measured every 15 min to avoid hypoglycemia.

Endogenous peroxidase activity was blocked with a blocking solution (Peroxidase block, EnVision™ Detection System, Dako, Carpinteria, CA, USA) for 15 min. To avoid non-specific unions, the tissue slides were exposed for 20 min in BSA.

Assays were run on an Mx3000P thermocycler (Stratagene) as follows: 95°C for 10 min, and 40 cycles at 95°C for 151s followed by 60°C for 1 min. To avoid any miRNA degradation, RNA extractions, reverse transcription reactions and real-time runs were all performed on the same day.

Proteins were digested with 20 μg of proteinase K (#AM2548; Ambion) in the presence of 40 mM Tris HCl pH6.5, 10 mM EDTA at 42°C for 45 min. To avoid genomic DNA contamination, samples were treated with DNase I for 30 min at 37°C.

Cell surface bound bacteria in both aliquots (before and after 1 hour incubation at 37°C) were detected by incubation with RPE labeled goat F ab' 2 fragments of anti-mouse IgG at 4°C for 30 min. To avoid eventual nonspecific binding of antibodies, all incubations were done in the presence of 25% heat-inactivated human serum.

The mice were allowed to explore objects for 5 min. To avoid possible preferences for one of two objects, the choice of the new and old object and the position of the new one were randomized among animals.