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Cultures were chased at 37 °C for 60 min to allow internalization of PrPC.
Subjects then rested quietly in a slightly darkened room for at least 45 min to allow for BP equilibration.
fumigatus sample and grinded for 10 15 min to allow the radical solution to penetrate into the cell walls.
The cells were incubated for at least 10 min to allow the CTV reagent to undergo acetate hydrolysis.
All injections were followed by an additional 2 min to allow diffusion before removal of the injection needle.
The devices were incubated within a humidity chamber at room temperature for 20 min to allow gel polymerization before filling the media channels with EGM-2.
Mice were rested for about 10 min to allow solidification of Matrigel before application of Op-site Flexigrid (Smith and Nephew) over the gel.
After 10 min incubation the cells were washed twice and incubated for 30 min. to allow the accumulation of oxidized mitoSOX in the mitochondria.
Virus was injected (1.0 μl, 100 nl/min) over 10 min, followed by an additional 10 min to allow diffusion of the viral particles.
Afterwards, the tissue was perfused with aCSF-HEPES for at least 20 min to allow for de-esterification of the dye before imaging experiments were commenced.
The homogenate was briefly mixed with a vortex and kept at room temperature (RT) for 5 min to allow nucleoprotein complex dissociation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com