Sentence examples for min to activation from inspiring English sources

Denuded oocytes were washed three times in Ca2+ free-NCSU23 medium [ 19] and then exposed for 5 min to activation medium consisting of 0.3 M mannitol, 0.05 mM MgSO4, and 0.1 mM CaCl2.

The mixture was stirred for 30 min to allow activation of functional group.

HT-29 cells were stimulated with epidermal growth factor stimulation of HT-29 cells for 30 min leading to activation of three MAPKs.

Participants will be seated for at least 5 min prior to activation of BpTRU.

The cells were preconditioned at each experimental environment for 60 to 90 min prior to activation with a concanavaline A (conA)/anti CD28 mixture (BD Biosciences) for T-cells or only with conA (Sigma) for the PBL.

Similar observations that ICI182,780 can change the time of ERK activation were reported in the case of a human thyroid carcinoma cell line, in which it reduced the 30 min to 1 hour activation, but enhanced later sustained activation at 6 hours [ 63].

To determine the effects of LPA and S1P on Akt and Erk phosphorylation under differentiation conditions, hNP cells were bFGF-starved for 24 hr and then treated with LPA or S1P for 10 min to assess Erk activation and for 30 min to assess Akt activation.

The cells were treated with 50 µM Forskolin for 20 min to induce cAMP/PKA activation and thereby lamellipodia and focal adhesion formation.

PKA- and ATP-stimulated single-channel currents were recorded for >5 min to ensure stable activation, following which MTSES was applied to the intracellular solution.

We extended the duration of DFMO treatment from 90 min to 4 days prior to activation of the cells.

All samples were collected by identical means, and serum samples were allowed to clot for 60 min to ensure thorough platelet activation before centrifugation and freezing.