Sentence examples for min to activate from inspiring English sources

Quantitative PCR was was initiated with an enzyme-activation incubation at 95°C for 15 min to activate DNA polymerase, followed by 40 cycles of denaturation at 95°C for 10 s and an annealing-extension step at 60°C for 1 min. For RNA viruses, RNA was first reverse transcribed.

Then, a 200 sccm H2 gas is flown for 10 min to activate the cobalt catalyst film.

A 1.2 equivalent amount DCC in DMSO solution was added to the PLGA/DMSO solution, which was stirred for 30 min to activate the carboxyl group of the PLGA.

This mixture was heated at 55 60°C for 10 min to activate arginase.

Prior to infection, the spores were heated to 65°C for 30 min to activate spores for germination.

The reverse transcription reaction was performed at 50°C for 30 min, and the mixture was incubated at 95°C for 15 min to activate Taq DNA polymerase.

Prior to the amplification, the samples were heated to 94°C for 10 min to activate the polymerase, and to denature the DNA.

K562-cells stably transduced with LAIR-1 and Jurkat-cells stably transduced with GPVI were incubated with FITC-labeled collagen for 20 min. To activate α2β1 receptor, CHO-cells expressing α2β1 were incubated in the presence of 1 mM MnCl2.

Prior to each experiment, the catalytic material was oxidized in situ at 450 °C for 1 h at an air flow-rate of 5 cc/min to activate the surface of the sample.

Cycling parameters were 95°C for 15 min to activate the DNA polymerase, then 40 cycles of 95°C for 15 s and 60°C for 1 min. Lipids were extracted as described [75].

Generally, CTAB (1.0 g), 2 M NaOH (aq, 3.5 mL), and H2O (240 mL) were mixed and heated at 80 °C for 30 min to activate the template.