Dissociation of Pri1 and Pri2 from the two early origins coincided with a further increase of DNA bulk synthesis at the 60 min and 75 min time points.
The aliquots were taken at 0, 10, 20 and 40 min time points and dephosphorylation reactions were stopped by 1% formic acid (pH 4.0).
HeLa-TRPV4 cells were harvested and plasma membrane proteins purified at 0, 3, 10 and 30 min time points under GSK101 stimulation.
Under basal conditions, LASP-1 strongly co-immunoprecipitated with HA-CXCR4 and upon stimulation with CXCL12, the association with LASP-1 mostly remained constant until 10 min, but was reduced by the 20 and 30 min time points.
To confirm the array data expression trends and peak times, a second biological replicate (second chemostat culture) was repeated for the 10, 30, 60 and 120 min time points, each also with a technical duplicate (dye-swap).
The similar measurements were done for peptide/phosphopeptide pairs from the D1, D2 and PbsH proteins and their flyability ratios were calculated using 0, 10, 20, 40 min time points (Table 1).
Mice were fasted for only 6 h, challenged with 2 g glucose/kg bodyweight, and blood glucose levels determined at 0, 7.5, 15, 30, 60, and 120 min time points.
At the 5- and 20-min time points after iohexol injection, the urinary system was imaged only vaguely, and after 60 min, only the urinary bladder was imaged.
The reduction of Tf uptake was observed from the initial 2- and 5-min time points (Fig. 1B).
The enhancement pattern changed only slightly between the 90- and 150-min time points after injection of the liposomes.
After stimulation with DEPs, we fixed cells in 4% paraformaldehyde at 0, 10, 20, 30, and 60-min time points.
