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The one hr of data collected was divided into three 20 min time bins to examine the dynamics of fights, which are known to change over the course of a fight [3].
Post hoc tests showed that KO mice had enhanced locomotor activity relative to WT (p<0.01), although Het mice did not differ from WTs. Analysis of novel open field data by 6 min time bins again revealed a significant genotype effect (F 2,279) = 12.8, p<0.001), as well as an effect of time (F 9,279) = 35.2, p<0.0001), and a significant genotype × time interaction (F(18,279) = 4.3, p<0.0001).
Data were collected in 10 min time bins during 1 h after all drug injections.
The time spent between the two threshold values was taken to represent locomotor activity in the mice and was collected in 10 min time bins over three consecutive phases: a 60 min initial habituation followed by saline injection and 60 min free exploration, and the third stage, 180 min of free exploration after intraperitoneal injection with 1 mg/kg AMPH.
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Two infrared beams crossed each cage and the number of beam breaks made per 5 min time bin was recorded during a 2 h test session.
The first hour of testing was analyzed separately in 10-min time bins, in order to evaluate exploration of the novel environment and locomotor habituation (Fig.5A, B).
Analysis of beam break activity divided into six 20-min time bins (ANOVA: Lesion Group3 × [Time Bin6 × S36]) revealed a main effect of time bin, F 5, 165) = 123; p <.0001, but no Lesion Group × Time Bin interaction, F(10, 165) = 1.5, p =.1.
Total beam breaks were recorded in 5 min time-bins over a period of 15 min.
Locomotor (distance traveled), rearing (vertical beam-breaks), stereotypical activities (repetitive beam-breaks <1 s), and anxiety level (duration in center area of arena) were measured in 5-min time-bins.
The number of beam breaks was recorded over a 60-min period, separated into 5-min time bins.
(T_{i}^{mathrm{bins}}): Series of time bins.
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