Sample washing was performed for 10 min three times unless otherwise noted.
Then, they were washed with PBS at 300 × g for 5 min three times.
They were treated with anodal right prefrontal and cathodal occipital TDCS (intensity: 2mA, time: 20 min, three times a week for four weeks).
After these processes, the excess PVP and EG were removed by adding deionized water centrifuged at 14,000 rpm for 10 min, three times.
The coverslips were then finally washed with PBS for 5 min three times and mounted onto glass slides after adding a drop of antifade solution.
Then, supernatant was collected for UV Vis measurements and Ag NPs were washed with water, using centrifugation (4000 rpm, 30 min), three times.
ORFV was added to the OFTu cells, and the cells were gently shaken for 10 min three times before the liquid was removed.
The unlinked AlC4Pc molecules were separated from AuNRs@SiO2 NPs after synthesis completion via centrifugation at 7,500 rpm for 30 min three times.
Spark sorted 100 TB on 206 nodes in only 23 min, three times faster with one tenth the number of machines.
Finally, the membranes were washed for 10 min three times.
For whole mount immunostaining, either fixed or cryoprotected embryos were equilibrated in 1×PTw for 10 min three times, and then subjected to the heating procedure.
