The electrochemically synthesized bicarbonate-doped PPy gave satisfactory potentiometric properties as pH-indicator electrode: sub-Nernstian response with a slope, m, of −39.06 mV pH−1 at buffer solutions of pH 3 10, excellent linearity, r, of −0.999, and relatively fast response time, average tr=3.6 min for three replicates (n=3).
For each sample, 2.5, 5.0 and 7.5 μl aliquots of tomato leaf extract were mixed with 2 ml ABTS radical cation solution and each reaction was kinetically monitored at 734 nm for 6 minutes with absorbances recorded at 1, 3 and 6 min. Three replicates were done for each aliquot volume.
Probe match results obtained using the Ribosomal Database Project (Cole et al. 2014) verified that the qPCR primer designed to target the 16S rRNA gene of S. aureus also targeted 77 sequences from Enterococcus.> -wrap-foot> Mean threshold time (T t, min) for three replicate reactions is listed with standard deviation.
The experimental design was a randomized complete block in a 2x7 factorial arrangement involving two cultivars (BR16 and EMBRAPA48) and seven water deficit periods (0, 25, 50, 75, 100, 125 and 150 min), respectively, with three replicates.
A non-sib male was immediately introduced into the tube and the occurrence of mating was recorded during a period of 30 min. Thirty two replicates per experimental treatment were conducted (leading to 96 females tested within 4 days).
Radial absorbance scans were collected in continuous scan mode at 280nm every 2 min with two replicates and a step size of 0.003 cm.
The effect of the length of the incubation time was studied using 150 μg of beads and eight different incubation times (1, 5, 10, 15, 20 or 30 min) with four replicates of each (n = 4).
Four typical volatile peaks were recorded every 4 min for all the three replicates.
Take Zika virus NS5 for three replicates, wherein two peaks were at 23 min and another peak was at 20 min.
To evaluate thermostability, three replicates of sonicated suspensions were maintained at RT, 50°C, 60°C or 70°C for 20 min before being transferred to ice and analyzed for activity.
