Sentence examples for min this was from inspiring English sources

48 min: This was just a bit sad.

Cogen session 25 min This was the first of the two cogen sessions.

The p-Si substrate was put on the hotplate at 150 °C for 5 min this was repeated three times.

However, the PCR was performed with slight modifications in the conditions, with an initial denaturation at 95°C for 5 min; this was followed by 35 cycles of 94°C for 5 min, 60°C for 30 s and 72°C for 1 min, and a final extension of 72°C of 5 min occurred.

Whilst most men in the current investigation undertook sufficient activity to meet the guidelines that recommend a specific volume of physical activity (assessed on either a minute-by-minute basis or in bouts of 10 min), this was not adequately distributed over the week to meet either the four day or five day aspect of different recommendations.

Mean awake minutes at night was 7.89 (95% CI, 7.14 8.63; range, 1 60 min), this was not age-related.

GP5+/GP6+ primer set: denaturing step of 95°C for 5 min, followed by 40 cycles of 95°C for 1 min, 40°C for 2 min and 72°C for 1.5 min. This was followed by a final extension period of 10 min at 72°C.

The gradient started from 60% B to 70% B in 20 min, followed by 70% B to 80% B in 10 min. This was held for 20 min followed by an increase to 95% B in 5 min. Again, this was held for 5 min before returning to starting 60% B, held for 20 min for column equilibration.

Cycling conditions were 94°C for 2 min followed by 35 cycles of 94°C for 1 min, 60°C for 30 sec and 72°C for 1 min. This was followed by a 4 min extension at 72°C.

Given that an scImpβ·cargo complex projects to 80 nm, this indicates that every point on the gel surface had been perforated more than 3000 times within 30 min or 100 times per min. This was essentially performed as previously described (Frey and Görlich, 2007).

The sections were rinsed, and then endogenous peroxidase was blocked with 0.3% hydrogen peroxide in Tris-buffered saline (10 mM Tris/HCl, 140 mM NaCl, pH 7.4) for 10 min. This was followed by 30 min of incubation with a biotinylated antirat IgG secondary Ab (Vector, Burlingame, CA, USA).