Sentence examples for min then the from inspiring English sources

Cultured cells were stimulated with 500 μM H2O2 for 10 min, then the media were removed and cells were rinsed three times with PBS.

The samples were centrifuged at 12 000 × g for 10 min at 4 °C followed by centrifugation at 100 000 × g for 30 min; then the supernatant, which contained the MTDs, was collected.

The product was collected and washed several times using the centrifuge (4000 × rpm for 10 min); then, the ZnO S.

In the study, rat eyes were enucleated immediately after death and perfused with a tracer (Evans blue) for 40 min. Then, the anterior segment was dissected and flat-mounted on a Petri dish with TM facing upwards.

Typically, the setting of the plaster started around 7 min after pouring the mixture and completed after 30 min, then the cast was separated gently from the tray.

Therefore, if we choose β such that β > 1 + 1 min i x i min, then the exponential utility can still be applied.

The extracts were prepared by brief vortexing and sonication for 15 min. Then the extracts were centrifuged for 15 min at 3500 rpm and dehydrated using anhydrous Na2SO4.

Ultra-thin sections were cut in an ultramicrotome, stained with 2% uranyl acetate for 15 min and 3% lead citrate for 3 min. Then the sections were observed under a transmission electron microscope.

The extracts were prepared by brief vortexing and sonication for 15 min. Then the extracts were centrifuged for 5 min at 13,000 rpm and filtered through 0.2 µm inorganic membrane filters (RC4, Sartorius, Germany).

The growth lasted for 40 min. Then, the whose system was cooled to 25°C.

The mixture system was stirred for 30 min. Then, the transparent solution formed was separated into two parts.