Sentence examples for min then for from inspiring English sources

The procedure comprised three phases: the lamb stayed alone for 2 min, then for 2 min with the familiar stockperson, and then alone for 2 min again.

After cooling for 20 min, they were treated with Triton 0.5% (SigmaX100) in phosphate buffered saline (PBS) for 30 min then for 3 min with pepsin (0.43 U/ml in 0.1N HCl, Sigma).

The solution was incubated on ice for 10 min, then for 10 min at 30°C.

Finally the substrate was washed by immersing it twice in Milli-Q water at 29 °C first for 20 min then for 19 h.

The reaction was carried out at 94°C for 5 min, then for 30 cycles at 94°C for 30 sec, 56°C for 30 sec, and 72°C for 1 min, followed by 72°C for 10 min.

The reaction was carried out at 94°C for 4 min, then for 30 cycles at 94°C for 1 min, 60°C for 2 min, and 72°C for 2 min, followed by 72°C for 10 min.

PCR conditions were as follows: initial denaturation 94°C for 15 min then 94°C for 30s 60°CC for 30s 72°CC for 30s repeated 35 times, final elongation 72°C for 10 min.

Reactions were in duplicate with incubation at 50 °C for 2 min, then 95 °C for 10 min, and then for 50 cycles of 95 °C for 15 s and 60 °C for 1 min.

The cycling parameters were 45°C for 2 min, 95°C for 1 min, then 95°C for 5 s and 60°C for 30 s for a total of 40 cycles.

PCRs were performed as follows: 95°C for 1 min, then 95°C for 1 min, 55°C for 1 min and 65°C for 8 min for 30 cycles.

The PCR cycling conditions were standard, 95°C for 10 min, then 95°C for 15 s and 60°C for 1 min for 40 cycles.