They were then switched to He O2 and observations repeated after 15 and 60 min and then every 60 min for a maximum of 360 min. The trial was terminated when no further change in the partial pressures of arterial oxygen (PaO2) and carbon dioxide (PaCO2) were scene.
MEXX was administered 60 min before the trial, and scopolamine was injected 30 min before the trial.
The mouse was placed into the water and left for 6 min. The time spent floating was measured during the final 4 min of the trial.
Furthermore, findings from the CAPTIM substudy offer little value in substantiating a recommendation of 'FMC to PPCI' of <60 min, when the 'FMC to PPCI' delay must have been at least 100 min in the trial.
During the acquisition phase, after the start chamber was lifted, the mouse was allowed to explore the maze for 3 min. The trial ended when the mouse entered the escape box or after 3 min had elapsed.
In the same hemodialysis conditions, the blood samples are dialyzed for 60 min, and the trial of each rate was repeated 3 times.
The weight was still increasing after 70 min in the trial, as depicted in Fig. 7a, indicating that water absorption was continuing.
The speed was slowly increased from 10 to 40 rpm within a period of 2 min. The trial was ended if the animal fell off the rungs or gripped the device and spun around for 2 consecutive revolutions.
Each mouse was administered imipramine hydrochloride (20 mg/kg, dissolved in saline) or vehicle i.p. 30 min before the trial.
