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In 40 min, the total average absorption volume was 267.64.
After centrifugation at 10,000 rpm for 5 min, the total DNA was extracted with a genomic DNA extraction kit (TaKaRa, Dalian, China) according to the supplier's instructions.
From 1 to 5 min, the total thickness of the coatings increases gently and the total growth rate declines sharply; at this stage, the outward growth gradually slows down, and the inward growth maintains persistent enhancement.
93 min later, the cellular topography becomes steady and no obvious change was observed in the next 63 min; the total cell height is raised but the cellular surface is smooth (Figure 3l-p).
On the other hand, in the countryside where the time interval between train operations is more than 10 min, the total amount of residual regenerative energy is not enough for the investment of energy storage system installation.
The total ischemic time of the whole liver was 30 min, the operative time was 251 min, the total blood loss was 240 ml, and the intraoperative blood transfusion was 280 ml of red blood cells (two units) and 240 ml of fresh frozen plasma (two units).
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A gradient was used and is described as follows: 10% B at 0 min and hold for 0.2 min, linear gradient to 95% B at 0.8 min and hold until 1.2 min, back to 10% B at 1.25 min and hold until 2.0 min. The total run time was 2.0 min, and the flow rate was 0.75 mL/min.
Results indicated that when the dwell time at peak load was less than 5-min, the total strain was mainly produced by the ratcheting strain due to the anelastic creep recovery and eventually failed due to ductile-fatigue damage, while for a longer holding time the accumulated creep strain and the fatigue-creep damage were the controlling mechanism of deformation and failure.
The gradient started from 65% A/35% B, reached 100% B in 6 min and remained there for the next 7 min. The total run time including a 5 min re-equilibration step was 18 min. The flow rate was 0.200 ml/min and the injected amount 0.75 µl.
The distinguishable difference in fluorescence emission among the clones began after 60 min and remained until 180 min. The total protein extracted from the induced T. thermophila clones at 180 min was analyzed by western blotting.
The gradient started from 65%A/35%% B, reached 100% B in 6 min and remained there for the next 7 min. The total run time per sample including a 5 min re-equilibration step was 18 min. The temperature of the sample organizer was set at 10°C.
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