Sentence examples for min the tissue from inspiring English sources

After incubation with normal rabbit serum for 30 min, the tissue sections were removed by blotting.

After washing in PBS (3 × 10 min), the tissue was mounted on glass slides using phosphate-buffered glycerol (pH 8.2) and examined under an Olympus fluorescent microscrope.

After a new wash of 3 × 30 min, the tissue was dehydrated using a series of graded acetone and finally embedded in spurr.

After 35 min, the tissue surface is exposed in air gradually with the agent penetrating, thus the water loss happens again.

After the endogenous peroxidase activity was blocked with methanol containing 3% hydrogen peroxide (Dako) for 30 min, the tissue sections were incubated with FSCN1 antibody or MMP14 antibody at 4 °C overnight.

After being blocked with rabbit serum (Vector Laboratories, Burlingame, CA, USA) for 30 min, the tissue sections were immunostained with the primary antibody, a rabbit monoclonal anti-insulin (1 : 200, Santa Cruz Biotechnology, USA).

The levels of ROS in brain tissues were determined by adding 2′, 7′ dichlorofluorescin diacetate to a final concentration of 10 μM in brain tissue homogenate and by incubating at 37 °C for 30 min; the tissues were then subjected to analysis using flow cytometry.

Considering the relationship between the assay sensitivity and incubation time as well as influence of flow rate (Figure 2D), the capture antibody coated beads were incubated for 60 min with the tissue lysates, for 60 min with biotinylated detection antibody mix and subsequently for 45 min with the reporter molecule Streptavidin-PE (each 1 µL).

For antigen retrieval, the sections were heated in 10 mM sodium citrate buffer (pH 6.0) at a sub boiling temperature for 10 min followed by cooling for 30 min. The tissue sections were incubated with mouse anti-CD45 (1∶200), rabbit anti-C3 (1∶100) or rabbit anti-Ccl2 (1∶100; Chemicon) diluted in 5% BSA in PBS for overnight at 4°C.

Briefly, sections were fixed in PBS containing 1% paraformaldehyde for 10 min, followed by 2 washes in PBS for 10 min. The tissue was rendered permeable in ethanol: acetic acid (2∶1, vol∶vol) at −20°C for 5 min, incubated with Image-iT FX signal enhancer for 30 min at room temperature (RT), and washed in PBS.

Pre-treatment heating was carried out in the pre-treatment buffer (Zymed Laboratories Inc). at 96°C for 15 min. The tissue was digested with pepsin for 10 min at room temperature, subsequently it was washed with deionised water, dehydrated with graded ethanol and air dried.