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In the next 3 min, the time spent investigating each of the objects was scored.
After 5 min, the time required for the deposition of the HMM molecules on the fibre surface, the unbound HMM were washed out with ATP-free buffer A plus 0.5 mg ml−1 bovine serum albumin (BSA).
Bilateral carotid occlusion with systemic hypotension was initiated for 10 min. The time to onset of the DC shift was recorded.
The calculated TIAC, however, is ascribed to heart wall based on the assumption that little activity would be in the blood after 45 min, the time of imaging.
After a period of 45 min, the time it takes for the cloud to travel the distance between the ACE satellite and Earth, the storm begins on Earth with an SSC, seen as a sudden increase in the H-component.
A full session with all the tests took an average of 75 min (the time on task, or "instructed content" lasted on average of 50 min in the first three conditions and 57 min in the final narrative condition).
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Naturally, if the oCelloScope system was set up to analyse every 5 min, the time-to-result would have been shorter.
The analytical column was first equilibrated in 98% A and 2% B. The linear gradient started with 2% B at 10 min (the time-point of valve switching), increasing to 40% B at 130 min, followed by 90% B from 135 min until 140 min, then 2% B from 142 to 165 min to prepare the system for the next injection.
Mice were placed on a rotating rod (32 mm diameter, O'hara), which initially rotated at 4 rpm, and then was accelerated at a constant rate from 4 to 40 rpm over 4 min, with the final speed maintained for 1 min. The time spent on the rotarod was measured in 3 trials every day between 1 00 PM and 7 00 PM and averaged.
After the volunteer had left the chamber, the door remained open and the monitoring of VOCs was continued for an additional 15 min. In total, a single experiment lasted 105 min. The time resolution of the measurements amounted to 30 s.
All patients underwent computer-assisted treadmill exercise tests, symptom limited, using the Bruce protocol, with a recovery phase of 6 min. The time interval between the consecutive tests was 30 min. We used a MAT2100 treadmill and a Fukuda Denshi ML8000 Stress Test system (Fukuda Denshi; Bunkyo-ku, Tokyo, Japan).
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