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After a quick purge of Ar (~ 20 min), the temperature was quickly ramped to 1,000 °C in 1 h.
After an initial stabilisation at 25 °C for 5 min, the temperature was increased by a 1 °C step every minute until 95 °C.
After system stabilization for 30 min, the temperature deviation did not exceed 0.2 K and the temperature was fully stabilized in less than 3 h.
A factory nanogrinder was operated for 15 min; the temperature increased from 44.25 °C to 46.25 °C and the relative humidity decreased from 47.5%to36.1%1%.
The flow rate was 0.8 min, the temperature 30°C and the detection wavelength 210 nm.
After degassing at 120 °C for 30 min, the temperature was raised to 230 °C.
Similar(35)
For β-actine, after an initial denaturing at 94°C for 5 min, the temperatures used were 94°C for 1 min, 56°C for 45 s and 72°C for 1 min followed by an extension of 5 min at 72°C.
For TNF-α, after an initial denaturing at 95°C for 5 min, the temperatures used were 95°C for 30 s, 57°C for 30 s and 72°C for 2 min followed by an extension of 5 min at 72°C.
The gradient started from 65%A/35%% B, reached 100% B in 6 min and remained there for the next 7 min. The total run time per sample including a 5 min re-equilibration step was 18 min. The temperature of the sample organizer was set at 10°C.
The PSi nanoparticles exhibited a very rapid increase in temperature for the first 1 min and a slow increase for the next 5-6 min. The temperature reached at approximately 70°C in about 6 min upon NIR laser irradiation and did not nearly changed thereafter.
At 60 min, the gelling temperature and melting temperature declined to 28 and 67 °C, respectively.
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