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After 60 min the solution was filtered.
After reaction for 10 min, the solution was centrifuged for 2 min at 12,000 rpm.
In 15 min, the solution was cooled and stored at 4 °C for a long term.
After N2 purging for 10 min, the solution was used for electrodeposition.
After reaction for 3 min, the solution was centrifuged at 12,000 rpm for 2 min.
After being kept in the dark for 30 min, the solution reaches the absorption-desorption equilibrium.
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The assembly mix was incubated at 60 °C for 5 min, 4 °C for 5 min, and finally 50 °C for 60 min. The solution was then dialyzed and electroporated into E. coli BL21 DE3) cells.
The substance was diluted in 1 mL of methanol and vortex mixed for 1 min. The solution was centrifuged for 3 min at 13 200 rpm/min.
The colourless reaction mixture was stirred for 15 min. The solution was concentrated at room temperature (r.t).
Finally, 24 μL seed solution were added rapidly, followed by gently mixing for about 1 min. The solution was then kept in 25 °C water bath overnight.
Briefly, 8 10 µL of protein preparation were applied to glow-discharged, carbon-coated Formvar grids (Electron Microscopy Sciences, Washington, PA) for 10 20 min. The solution was gently wicked off using Whatman grade-1 qualitative filter paper.
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