Sentence examples for min the membrane from inspiring English sources

After several hours of operation (50 300 min), the membrane partially wetted mode is established at steady state and decreases the absorbed CO2 flowrates up to 10.5% compared to membrane dry mode.

As one voltammogram lasted about 2 min, the membrane deposition on one sensor took 6 min.

After 3x washes with TBST for 10 min the membrane was incubated for 30 min using a 1/1000 dilution of secondary anti-rabbit peroxidase antibody (Sigma-Aldrich Lot. A0545-1ML).

After drying for 15 min, the membrane was blocked for 1 hour with 3% BSA (Fluka) in PBS at RT.

During the first 20 to 30 min, the membrane conductance increased by several orders of magnitude above that of membranes without the protein (from about 0.05 µS/cm2 to 150 µS/cm2).

After washing twice with TBS-T for 20 min, the membrane was incubated with an appropriate HRP conjugated secondary antibody in Western milk, washed again (2×20 min in TBS-T) and imaged using ECL plus for chemifluorescence.

After washing in PBS (3 × 3 min), the membranes were incubated in a peroxidase-conjugated goat anti-rabbit IgG (Sigma; 1 400) in the blocking buffer for 2 h.

In the investigated ranges of t from 0.67min to 20 min, the membranes with cellular structure are obtained at both elevated Td and Tv of 65 °C, relative humidity of 70%RH and mass fraction of 15 wt% PVDF.

After a final centrifugation step at 48000 x g for 10 min the membranes were resuspended in 50 mmol/l Tris-HCl buffer, pH 7.4 and incubated with 2 U/ml adenosine deaminase (ADA) at 37°C for 30 min. The membranes were frozen in small aliquots and stored at −80°C until binding assay.

After again washing three times for 10 min, the membranes were incubated 2 times for 2 min in assay buffer containing 200 mM Tris-HCl and 10 mM MgCl2 (pH 9.8).

Non-invaded cells were removed and invaded cells were fixed in Carnoy's fixative for 15 min. After drying, the nuclei of invaded cells were stained with Hoechst 33258 (50 ng/mL) in PBS for 30 min, the membranes were washed, mounted and viewed with a Nikon eclipse TE300 fluorescent microscope.