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Meanwhile, when the ultrasonication time was longer than 45 min, the increase of the SEM of mean nanoliposome diameter occurred, indicating an increase of differences among the liposome samples.
Clotting was initiated with 0.1 U/mL human thrombin (HYPHEN BioMed) and monitored by absorbance at 600 nm every minute for 15 min; the increase in optical density corresponded with noticeable gel formation.
At 10 min, the increase was approximately four times.
We found that centrosomes in Tiam1-depleted cells separate significantly further 10 min after washout compared to controls; however, at 20 min the increase was not significant.
Thus, after 90 min the increase in intracellular fluorescent intensity was 200% for SWA11 mAb and 50% for L1-9.3 mAb.
Removal of HSPG by heparinase treatment led to a 2.1 fold increase in I-LDL transport at 10 min. At 15 min and 30 min the increase in LDL transport was 57% and 36% higher than controls.
Similar(52)
For example, after 3 min the increases are as much as 130 500%% (or factors of >1.3 5) compared to yields achieved with the ethanol-free, pure aqueous system.
The intensity of both peaks increases while the reactions progress, and after around 40 min, the increasing of intensity becomes extremely slow, indicating that the GNPs are formed and stabilized in the early 40 min.
After 30 min the increased transcription had resolved somewhat, corresponding with majority clearance of the DMNQ [24].
For NINs and NQA-MINs, the increase in zeta potential is probably due to nonspecific adsorption of template peptide.
For example, the time required to double the number of transcripts initiated from P1 was 1.28 min during the INCREASE period, 1.37 min during the STEADY-STATE period, and 5.2 min during the DECREASE I period.
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