Sentence examples for min the fluorescence from inspiring English sources

After NSPCs were incubated with 10  μM DCFH-DA at 37 °C for 30 min, the fluorescence was monitored by scanning the whole well using a fluorescent plate reader at excitation and emission wavelengths of 485 and 525 nm.

After the samples were irradiated by a visible light (400 to 440 nm) with a power density of 40 mW/cm2 for different times ranging from 1 to 5 min, the fluorescence spectra were recorded by a spectrometer F-25000, Hitachi, Brisbane, CA, USA) and the fluorescent intensities were compared.

When slices were treated with a calcium-free solution for 15 min, the fluorescence of the control group decreased by 61±4%4% (N = 5; data not shown).

After pre-incubation for 5 min, the fluorescence of AMC released from the digested peptides was measured on a fluorometer (Infinite® M1000, Tecan Trading AG, Mannedorf, Switzerland) every 1 min for 20 min (excitation of 380 nm and emission of 460 nm).

After the mixture was vibrated for 5 min, the fluorescence spectra of the mixture were recorded.

Within 20 min, the fluorescence intensity of the nanoparticle suspension decreased only slightly.

Cycling conditions for genotypings comprised an initial cycle at 60°C for 1 min and 95°C for 10 min, followed by 55 cycles at 92°C for 15 s and 60°C for 1 min, and a final step at 60°C for 1 min. The fluorescence intensity of was measured by the 7500 RT-PCR System (Applied Biosystems).

The PCR started with an initial step at 95°C for 10 min, followed by 40 cycles with denaturation at 95°C for 1 min, annealing temperature for 30 sec and elongation at 72°C for 1 min. The fluorescence was measured at the end of the annealing and at the end of the dissociation program at a wavelength of 530 nm.

Cycling conditions were 95°C for 10 min, followed by 45 cycles of 95°C (15 s) and 60°C (1 min) where the fluorescence was acquired.

The diluted mixture was then heated in a 100 °C hot-block for 5 min and plunged into a water bath at room temperature for 5 min before the fluorescence was measured again.

The cells were further incubated for 60 min, and the fluorescence level indicating intracellular ROS production was measured at 0 and 60 min using a fluorescence microplate reader (Infinite 200 PRO; Tecan Japan, Kanagawa, Japan; or Cytofluor 4000J; Applied Biosystems Japan) with λex at 485 nm and λem at 530 nm.