Sentence examples for min the flow from inspiring English sources

The fractions were collected every 3 min; the flow rate of the column was 1 mL min−1.

After the growth of Si1−x Ge x nanowires for 30 min, the flow of SiCl4 was terminated and then a flow of O2 was introduced at 900°C under vacuum maintained by mechanical pump.

The linear gradient was 0 60% B in 20 min and then 60 100% B in 5 min; the flow rate was 80 µL·min−1.

Then, after 20 min, the flow rate was decreased to 200 sccm until complete consumption of the carbon source.

After 2 min, the flow chamber was subsequently washed with TIRF buffer (See 'Materials and methods') to remove unattached filaments, free monomers, and GMPCPP.

After centrifugation at 4000 g for 7 min, the flow through was discarded and two washing steps with 10 mL RPE buffer (Qiagen, Germany) followed with centrifugation at 4000 g for 3 min and 10 min, respectively.

After reduction, the solution was centrifuged at 14,000g for 15 min, the flow-through was discarded and added 200 μL UA buffer and 5 μL 25 mM IAM, vortexed (600 rpm) for 1 min.

The solvent system was aqueous phosphoric acid (0.1% w/v; eluent A) and acetonitrile (eluent B), running as a gradient from 10% B to 27% B within 15 min, to 40% B within 45 min: the flow-rate was 0.5 ml/min and detection at 220 nm.

The total running time, including a 5 min re-equilibration step, was about 50 min. The flow rate was 1.0 mL min-1 and 10 μL samples were injected.

The oven was held at 160°C for 1 min, ramped to 310°C at 20°C min-1, and finally held at 310°C for 0.5 min. The flow rate was 1 mL min-1 and the split was 10.

The mobile phases were composed of acetonitrile (B) and 0.1% o-phosphoric acid (A) using a gradient elution of 95-90% A at 0 5 min; 90-80% A at 5 8 min; 80-0% A at 8 10 min; 0-95% A at 10 11 min. The flow rate was 0.3 mL/minute.