Sentence examples for min the extract from inspiring English sources

After stirring using a VWR Analog Vortex mixer for 35 min, the extract was decanted into another vial.

Even after autoclaving for 20 min, the extract was still capable of reducing the lesion diameter from 15 mm to 4 mm.

After exposure to 100°C in water bath for 20 min, the extract was able to reduce the lesion size caused by P. capsici on pepper leaves from 15 mm in diameter in the control to 2 mm.

This HM-SFE system was used for obtaining guava (Psidium guajava L). seed oil, using supercritical CO2 adding ethanol as co-solvent (CO2 SC/EtOH), extractions were performed at 313 K and different pressures (10, 20 and 30 MPa), each one in four stages of 30 min, the extract with higher yield was subjected to transesterification and high-resolution gas chromatography (HRGC) analysis.

After sonication for 10 min, the extract content was determined by UV spectrophotometry at 286 nm.

After incubation in ice for 20 min, the extract was centrifuged for 15 min in a microcentrifuge at a maximum speed at 4 °C and the supernatant (cytoplasmic extract) was loaded onto 10 30% linear sucrose gradient containing 30 mM Tris-HCl (pH 7.5), 100 mM NaCl and 10 mM MgCl2.

The nuclear pellets were washed with lysis buffer lacking NP-40, resuspended in 250 mM Tris HCl [pH 7.9], 100 mM KCl, 1 mM EDTA, 1 mM DTT, 0.5% NP-40, 20% glycerol and mixed on a wheel at 4°C for 30 min. The extract was then centrifuged at 10 000 rpm for 30 min at 4°C, the supernatant collected and used directly [38].

The extracts were then incubated with plasmid DNA (at a concentration of 130 µg/ml) on ice for 10 min. The extract was then centrifuged at 16 000 × g for 10 min to remove precipitations.

After 50 min the strips were dropped into 2 ml of boiling 50% ethanol and boiled for 2 min. The extract together with one further extract made with 2 ml of boiling ethanol was dried down and the residue was dissolved in a small volume of 50% ethanol and applied to a Whatman K2 cellulose thin layer (250 μm) chromatography plate.

A weighed quantity of the powder equivalent to 10.0 mg MTP and 1 mg of FDP (in their ratio of 10 1) was transferred into a small conical flask and extracted with 50 mL of methanol by ultrasonication for 30 min. The extract was filtered with acrodisc GHP (Gelman Hydrophilic Polypropy lene membrane) into a 100 mL volumetric flask.

After 30 min exposure, the extract still caused complete inhibition of conidial germination of A. brassicicola in comparison with the 97% germination in the water control.