Sentence examples for min the cell from inspiring English sources

After centrifugation at 1,000 rpm for 5 min, the cell pellet was washed with 1 mL ice-cold PBS again and spun down at 1,000 rpm for 5 min.

After being ultrasonically (Scientz-II D sonifier) disrupted at 225 × 4 s with cooling in between on ice for 15 min, the cell suspensions were centrifuged (10,000×g; 10 min at 4 °C).

The mixture was vortexed for 1 min, the cell suspension centrifuged (9 000 g, 20 min, 4°C).

After incubation on ice (20 min) the cell lysates were centrifuged for 10 min at 13000 rpm and supernatants were collected.

After non-dissociated tissue was allowed to settle for 3 5 min, the cell supernatant was centrifuged for 5 min at 350 g.

Following clarification by ultracentrifugation (100,000×g for 45 min), the cell lysate was applied on HisTrap chelating nickel affinity column (GE Healthcare).

After the cells were fixed with 4% paraformaldehyde in PBS at 25 °C for 15 min, the cell nuclei were stained with Hoechst 33258 for 15 min.

Treated groups were lysed after 10 or 60 min, the vehicle control at 60 min. The cell lysate was processed according to manufacturer's recommendations.

Cells were then incubated for 30 min on ice and sonicated (Brandson 1510) for 15 min. The cell suspension was passed through a 26-gauge needle and protein quantification was performed using the Bradford method.

After grinding with semifrosted slides and lysis of RBC, the dissociated cells were incubated on ice for 20 min, and then spun down at 500 rpm for 1 min. The cell pellet was washed and used as tumor cells.

Harvested cells were centrifuged at 1,500 g for 5 min, washed in phosphate-buffered saline (PBS), and pelleted at 1,500 g for 5 min. The cell pellet was rapidly frozen in liquid nitrogen and stored at −80°C.