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After incubation at 100°C for 15 min, the absorbance of the colored product was measured at 450 nm.
After 15 min, the absorbance was read at 517 nm.
After 30 min, the absorbance of solution is measured at 882 nm using a spectrophotometer.
After 3 min, the absorbance of the colored solution was measured at 500 nm.
After 15 min, the absorbance was measured at 593 nm [11].
After incubation at 37 °C for 20 min, the absorbance was measured spectrophotometrically at 415 nm.
After placing there for 20 min, the absorbance was recorded at 560 nm.
Similar(4)
The mixture was shaken and left at room temperature for 10 min. The absorbance was measured at 562 nm.
The plate was then incubated at 37°C for 60 min. The absorbance at 410 nm was measured by using a microplate reader Bio-Tek Instruments , Inc). Bio-Tek Instruments , Inc
The reaction mixture was then vortexed and incubated at room temperature for 30 min. The absorbance of the ferric-xylenol orange complex was measured at 560 nm.
After incubation at room temperature for 30 min, the absorbance of the reaction mixture was measured at 415 nm.
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