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At the end of behavioural testing, a two-day forced swim test with 6 min sessions was performed as previously described ([ 38, 41]; for scheme of study flow, see Figure 1(b)).
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Ten consecutive 30 min sessions were conducted.
Both 15 min sessions were video recorded to facilitate scoring of all identifiable distinct behaviours.
The pegboard work was interrupted every 5 min for inquiring about pain intensity (VAS) and rate of perceived exertion (RPE), thus a total of 8 × 5 min sessions were performed.
Briefly, mice were pretested in the apparatus, and time spent in each compartment during the 30 min session was video recorded and analyzed using Ethovision (Noldus).
Moreover, the total distance moved during the 10 min session was analyzed and the number of fecal boli dropped was counted at the end of each trial.
In addition, the total distance moved during the 10 min session was analyzed and the number of fecal boli dropped was counted at the end of each trial.
A 60 min session was allotted for survey completion.
min after administration of test compounds, mice were put on the rotating rod and latency until fall during the 5 min session was recorded.
The locomotor activity that we observed in vehicle-treated rats during the 10 min session was consistent with previously reported independent results for the same strain.
Mice were returned to the chamber 24 h later, and the percent time freezing over the 3 min session was examined.
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