Sentence examples for min separated by from inspiring English sources

Nuclei were prepared by two 500 g centrifugation steps, each 5 min, separated by a 5 min wash in cold PBS.

Protein samples were denatured in Laemmli buffer, boiled at 95 °C for 10 min, separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes.

Plasma samples were mixed with 5x SDS loading buffer, boiled for 2 min, separated by SDS-PAGE, and transferred to nitrocellulose membrane (Millipore).

Total protein extracts were prepared by boiling the cells in 1.5× SDS sample buffer for 10 min, separated by SDS-Tricine-PAGE, and transferred onto Hybond ECL nitrocellulose membranes (Amersham).

Lysates were heated at 95°C for 5 min, separated by electrophoresis on a 10% polyacrylamide gel and transferred onto a polyvinylidene difluoride membrane.

Proteins were solubilized in sample buffer, heated at 95°C for 5 min, separated by SDS-PAGE on 8% polyacrylamide gel and transferred on a 0.2 µm nitrocellulose membrane.

These laboratory-based studies compared a light intensity standing/walking group to a sitting group, employed LIPA (14 sessions of 2 min LIPA separated by 20 min sitting period) dispersed throughout the day, and measured postprandial glucose.

Beginning on the third day, to the termination of the experiment, colony entrances were blocked with wire-mesh for 15 min intervals separated by at least 30 min to enable the capture of returning focal foragers.

Animals were subjected to a series of 1-min sessions separated by 10 min on the rotarod apparatus (ROTA-ROD/RS, Letica Scientific Instruments, Panlab, Barcelona, Spain) using the following protocol: 0 r.p.m., 4 r.p.m., three sessions of accelerating 4 40 r.p.m. (two training sessions followed by the test session).

The pellets were then incubated with buffer A for another 15 min and separated by centrifugation at 12 000 g for 5 min at 4°C.

During each session, cocaine was available during 6-min periods, separated by 26-min no-drug periods.