Sentence examples for min room from inspiring English sources

The peptidoglycan fraction was pelleted by centrifugation (21,000 × g, 20 min, room temperature), washed and resuspended in water.

Cells were fixed in 4% Paraformaldehyde (PFA, 20 min, room temperature -RTo), washed with PBS and permeabilised with 70% EtOH (overnight (o/n), 4 °C).

Then, the tube was cooled for 1 h, and centrifuged for 5 min (room temperature).

The insoluble material was removed by centrifugation (7800 g, Sorvall RC 3C Plus, Thermo Scientific, 30 min room temperature).

DCs were similarly treated with EDTA (30 min room temperature).

The reaction was quenched by adding 15 µl of 4 M hydroxylamine (15 min, room temperature in the dark).

Cells were harvested by centrifugation (500 g, 10 min, room temperature) and directly resuspended in 3 vols 6.6%% HClO4.

Plates were rinsed and flicked 3x, blocked (5% milk), and exposed to human diluted sera (90 min, room temperature).

Measurements were taken 24 h after the treatment following 30-min room acclimatization.

After a 30-min room temperature incubation, plates were read on a ViewLux plate reader (PerkinElmer) using a 20-sec exposure time.

After incubation for 30 min. (room temperature) the wells were washed three times and substrate (TRIS-puffer with p-Nitrophenylphosphate tablets) was added.