However, this radioligand has various limitations, among others, the labelling with C (T1/2: 20.4 min) restricts its extensive clinical use [ 2].
Prior to CTmin and CTmax determination, moths were chilled at 5°C for 5 min to restrict movement and allow individual placement into the chambers.
While SMURFLite with an interleave threshold of 1 or 2 requires roughly 1 s of wall-clock time on a 12-core 2.4 GHz AMD Opteron server, an interleave threshold of 4 raises this run-time requirement to 7 10 min. Restricting the interleave threshold to 3 or less has different impacts on the different folds in our test set.
C, the most commonly used nuclide, is produced by a cyclotron and has a short half-life of 20.5 min, which restricts the use to PET centers with an in-house cyclotron facility.
The program of thermal cycler was 5 min denaturation at 94°C followed by 38 cycle of denaturation at 94°C for 1 min, annealing at Tanneal (Table 1) for 1 min, and extension at 72°C for 1 min, with a final extension at 72°C for 5 min. Amplified restricted fragments were detected after digestion with BstBI, and AvaI was only used for Val/Met255 variant.
Transferred DNA was UV crosslinked and subsequently immobilized by baking at 120 °C for 30 min. Restricted and immobilized genomic DNA was then allowed to hybridize with DIG-labelled probes targeted against a hygromycin phosphotransferase coding sequence present towards the left border in T-DNA of the three binary vectors used to transform banana cells.
However, ever since the implementation of the Mandarin-only policy in 1946 in Taiwan, non-national languages such as Min have been largely restricted to private domains (Huang 黃宣範 1993), and even in this realm, Mandarin is gradually encroaching.
Beier and Lindauer (Beier 1968; Beier and Lindauer 1970) showed that the circadian rhythm is adjusted daily by the sun as the "Zeitgeber", and Koltermann (1971) showed that the "Zeitgedächtnis" lets the bees distinguish between time points as close as 20 min and is not restricted to odours but also applicable to colours.
The duration of exposure at the predefined levels of oculomotor load was restricted (7-min in each viewing condition) in the present study.
We collected new bats every 30 min to avoid restricting foraging opportunities for these individuals and to ensure continuous distress calls throughout trials.
Due to the long exposure to IL-1β (24 h) pre-stimulation was restricted to 45 min in these experiments.
